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Báo cáo y học: "Genome-wide promoter extraction and analysis in human, mouse, and ra" potx
... National Center for Biotechnology Nucleic Acids Res 2003, 31:28-33 Brudno M, Do CB, Cooper GM, Kim MF, Davydov E, Green ED, Sidow A, Batzoglou S, NISC Comparative Sequencing Program: LAGAN and Multi-LAGAN: ... be downloaded from our FTP site at [37], the promoter set for Affymetrix array HG-U133A is in [38], the promoter set of all RefSeq genes is in [39], all known promoters in CSHLmpd can be downloaded ... accessibility Nucleic Acids Res 2004, 32:W191-W194 Coleman SL, Buckland PR, Hoogendoorn B, Guy C, Smith K, O'Donovan MC: Experimental analysis of the annotation of promoters in the public database...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... Vanilloid receptor VR1 transiently transfected in the rat dorsal root ganglia derived cell line F-11, a hybridoma of mouse neuroblastoma and rat dorsal root ganglion cells [6] VR1 expressed in a neuronal ... anti-VRL-1 Western blotting Incubation with Endo H abolished the upper band of the anti-VRL-1 immunoreactivity doublet band at > 80/84 kDa The lower band of the doublet at 80 kDa increased proportionally ... CTGGAGGACCTGA-3¢) The five introduced nucleotides are shown in bold, they generate a new codon for P761 and a stop codon The accuracy of the resulting new plasmid pcDNA3.1/rVRL-1 was confirmed by DNA sequencing...
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Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt
... Additionally, a random probe was used as negative control (data not shown) Microphotographs were scanned, digitally processed and compiled using computer imaging software (Adobe PHOTOSHOP 5.5 and Adobe ILLUSTRATOR ... systems in different species and developmental stages Brain tissue has been studied by this methodology predominantly at developmental stages In one recent study, however, expression of ADH1 and ADH4 ... other ADH classes After this iterative process, for example our chosen rat class ADHprobe (rADH3) does not show significant similarity to rat class I ADH or IV mRNAs or to any other rat mRNA as determined...
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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... glycosylation is impaired in PS1deficient cells, and down-regulation of NCT inhibits c-secretase cleavage of APP and Notch [11–13] PEN-2 is a small double membrane-spanning protein with N- and C-termini ... facing the lumen [14] APH-1 contains seven putative transmembrane domains and has a variety of isoforms with differing C-terminal domains; in humans, APH-1a long and short forms are encoded by a ... intermediate glycosylated form of NCT ( 120 kDa) than adult brain where the predominant NCT species was a 140-kDa form NCT ectodomain Ab 00/22 reactivity indicated detection of only the less mature or...
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báo cáo hóa học:" Tissue specific characteristics of cells isolated from human and rat tendons and ligaments" pptx
... between ligaments and tendons of the ovine hind limb It is well documented that there are functional differences between tendons depending on their role in the body Tendons can be classified according ... environment of a tendon varies greatly both within and between tendons [46] and is also likely that the cellular make-up of a tendon or ligament reflects this Both the patellar tendon and ACL are ... tendons and resident cell populations to glucocorticoids In vitro studies on the effects of corticosteroids on cultured tendon/ligament cells are also bedevilled by varying conditions of dosage,...
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Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx
... cotton rat (Sigmodon fulviventer and S hispidus) Proc Natl Acad Sci USA 1998, 95:14355-14360 Rytik PG, Il'kevich Iu G, Poleshchuk NN, Miuller V, Kucherov IA, Duboiskaia GP, Podol'skaia IA, Novikova ... Previously, we and others [16-19] have shown evidence of HIV-1 infection in two cotton rat species (Sigmodon hispidus and S fulviventer) In one study [16] cotton rats inoculated with HIV-1 developed detectable ... and hCXCR4 (K4) was also established using pCDNA3.1 expression vectors (Fig 1E) The proportion of double expressor cells in the population of this clone was ~32% during early passages and this proportion...
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Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
... cotton rat (Sigmodon fulviventer and S hispidus) Proc Natl Acad Sci USA 1998, 95:14355-14360 Rytik PG, Il'kevich Iu G, Poleshchuk NN, Miuller V, Kucherov IA, Duboiskaia GP, Podol'skaia IA, Novikova ... Previously, we and others [16-19] have shown evidence of HIV-1 infection in two cotton rat species (Sigmodon hispidus and S fulviventer) In one study [16] cotton rats inoculated with HIV-1 developed detectable ... and hCXCR4 (K4) was also established using pCDNA3.1 expression vectors (Fig 1E) The proportion of double expressor cells in the population of this clone was ~32% during early passages and this proportion...
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Báo cáo y học: " Biphasic effect of extracellular ATP on human and rat airways is due to multiple P2 purinoceptor activation" ppsx
... using the Ca2+-sensitive probe indo-1 as previously described [18] Briefly, freshly isolated cells were loaded with indo-1 by incubation in PSS containing µM indo-1 AM for 25 at room temperature ... thapsigargin were purchased from Sigma (Saint Quentin Fallavier, France) Indo-1 AM was from Calbiochem (France Biochem, Meudon, France) Indo-1 AM and thapsigargin were dissolved in dimethyl sulphoxide which ... prostanoid release [25] Such an effect does not seem to be involved in rat IPB, since the return to baseline was not modified by indomethacin However, indomethacin did prolong the contractile...
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Báo cáo y học: " Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages" docx
... examined p24 production by CD4+CD8- T cells prepared from double Tg rats in comparison to WT rat and human cells CD4+CD8T cells prepared from double Tg rats produced p24 in the medium approximately ... from double Tg rats or human PBL, and the medium was recovered days after infection Values are the means of triplicate samples phages and subsequently infected the cells using HIV-1 pseudotyped ... cells transiently expressing untagged hCRM1, but not rCRM1 (Fig 1B) These results suggest that endogenous rCRM1 supports p24 production less efficiently than the hCRM1 and that Rev function is...
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Báo cáo y học: "TFCat: the curated catalog of mouse and human transcription factors" pdf
... helix-turn-helix 16 Ets domain family 23 23 1.2 Winged helix-turn-helix 101 GTF2I domain family 12 1.2 Winged helix-turn-helix 102 Forkhead domain family 26 26 1.2 Winged helix-turn-helix 103 RFX domain family ... Nagadoi A, Nakazawa K, Uda H, Okuno K, Maekawa T, Ishii S, Nishimura Y: Solution structure of the transactivation domain of ATF-2 comprising a zinc finger-like subdomain and a flexible subdomain ... were added to the system to map domain structures (Table S5 in Additional data file 1) The DNA binding domain analysis offers some noteworthy observations The homeodomain-containing genes are prominently...
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Investigation of relative expression level of SLC4 bicarbonate transporter family in mouse and human corneal endothelial cells
... preservation to RNA extraction days Primary endothelium Sample Primary endothelium Sample Primary endothelium Sample Primary endothelium Sample Primary endothelium Sample Cultured Cells Sample Cultured ... have in common three structural domains: an N-terminal hydrophilic, cytoplasmic domain, a hydrophobic, polytopic transmembrane domain, and a C- terminal cytoplasmic domain (Romero MF et al., 2004, ... in cultured (passage and 7) mouse corneal endothelial cells compared to the primary endothelium 50 Figure 3.7 Morphology of cultured human corneal endothelial cells (HCECs) 52 Figure 3.8 Cellular...
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Human rationality and artificial intelligence
... Associates, 1991) and in Cynthia Macdonald and Graham Macdonald (eds.), Connectionism: Debates in Psychological Explanation, Volume (Oxford: Blackwell, 1995) See Jerry A Fodor and Zenon W Pylyshyn, ‘Connectionism ... 11, (1988), pp 1–74, reprinted in Macdonald and Macdonald (eds.), Connectionism See also, in the same volume, the subsequent exchanges between Smolensky and Fodor et al 226 An introduction to the ... true Thus, both a situation in which Tom is in London and a situation in which Tom is in Paris provides a model of the premise ‘Either Tom is in London or Tom is in Paris’, but only one of those...
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The Modern Human colonisation and the Neanderthal extinction
... technology adopted by Moderns and Archaics across the world it is Modern Human colonisation and Neanderthal extinction 159 not unreasonable to expect behavioural responses such as those documented ... probably because of it (Van Peer, 1998) The degree of coincidence with the arrival of Moderns does leave the door open in respect of whether such behaviours arose independently or whether, instead, ... Neanderthals had entered previously They managed to hold out, just as populations of Neanderthals had done during earlier cold events, and subsequently they spread north (Torroni et al., 1998, 2001)...
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The revolution in philosophy (I) - human spontaneity and the natural order
... that Immanuel Kant, Critique of Pure Reason (trans N K Smith) (London: Macmillan and Co., ), xxxv, p Dogmatism is defined early in the Critique by Kant as “the presumption that ... for judgment under a concept; it does not put it in fully discursive conceptual form, nor bring it under a category – that can only happen in judgment – but it does not grasp it without any kind ... radical freedom of will that initiates a causal series but is not itself an effect of any other cause; and that there must be a cause for every event, and hence there can be no freedom This was,...
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The return of universalist law - human rights and free trade
... at times be referred to as ‘the Universal Declaration’), adopted by the United Nations General Assembly in 1948 Two other documents adopted in 1966 are commonly dealt with in the context of twentieth-century ... Klug, Values for a Godless Age: The Story of the United Kingdom’s New Bill of Rights (London: Penguin Books, 2000) This need not involve abandoning private beliefs that we have souls with different ... dispute resolution, the borrowing of federal constitutional doctrines such as 150 151 152 154 Tuna/Dolphin I, 30 ILM 1594 (1991); Tuna/Dolphin II, 33 ILM 839 (1994) United States – Import Prohibition...
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Tài liệu To establish an Office on Women’s Health within the Department of Health and Human Services, and for other purposes pdf
... focus in areas of importance to women; •HR 1784 IH 18 ‘‘(D) supporting the development of meth- odologies to determine how to obtain data spe- cific to women (including data relating to the age...
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Tài liệu NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Di-Isodecyl Phthalate (DIDP) pdf
... weight ratio in of dogs at the highest dose tested Swollen and vacuolated hepatocytes were noted in two mid-dose males, two mid-dose females, one high-dose male, and three high-dose females The ... administered dose that was recovered in bile decreased with increasing dose from 14% at a dose of 0.1 mg/kg to 4.7% at a dose of 1,000 mg/kg In rats exposed by inhalation, 45 and 41% of the absorbed dose ... increasing dose from 52% at the low dose to 72% at the high dose, while the phthalic acid decreased from 38 to 18% The monoester oxidized derivative, MIDP, and DIDP were all detected in feces in dose-dependent...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf
... LEDGF-interacting domain linked to DNA-binding domains (AT-hook and MT domain) becomes disconnected from the PHD domains, the FYRN domain, the transactivating domain, the FYRC domain and the SET domain ... binds to AF10 and DOT1L) Therefore, MLL–AF4, MLL– AF9, MLL–ENL and MLL–AF10 fusion proteins are all functionally equivalent as they all bind, directly or indirectly, to the DOT1L protein Because ... can be inhibited by the potent CDK9 inhibitor, flavopiridol, an experimental drug identified in 1992 as an anticancer drug [37] Flavopiridol has been tested in several clinical trials but was found...
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Tài liệu Báo cáo khoa học: Changes in purine specificity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx
... PDE GAF domains cAMP is the ligand for these cyanobacterial tandem GAF domains, and cAMP binding results in stimulation of the C-terminal catalytic domain [7–9] To date, two tandem GAF domains ... Anabaena tandem GAF domains probably requires structural changes in the b1–b3 region Because in the tandem GAF domain of cyaB2 cAMP is bound by both subdomains, we used the tandem GAF domain of cyaB1 ... cAMPÆmg)1Æmin)1 J U Linder et al Cyclic nucleotide specificity in GAF domains 1517 Cyclic nucleotide specificity in GAF domains J U Linder et al Fig Dose–response curves for cAMP and cGMP stimulation of cyaB1...
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